hoechst 33 342 (Dojindo Labs)
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Hoechst 33 342, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 384 article reviews
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Incubation:Article Title: CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction. Article Snippet: Although microglial activation is induced by an increase in chemokines, the role of mitophagy in this process remains unclear.. This study aimed to elucidate the role of microglial mitophagy in CKLF/CKLF1 (chemokine-like factor 1)-induced microglial activation and neuroinflammation, as well as the underlying molecular mechanisms following CKLF treatment.. This study determined that CKLF, an inducible chemokine in the brain, leads to an increase in mitophagy markers, such as DNM1L, PINK1 (PTEN induced putative kinase 1), PRKN, and OPTN, along with a simultaneous increase in autophagosome formation, as evidenced by elevated levels of BECN1 and MAP1LC3B (microtubule-associated protein 1 light chain 3 beta)-II. Article Title: Ido2 Deficiency Exacerbates Motor Impairment and Reduces Aryl Hydrocarbon Receptor Activity through Decreased Kynurenine in a Chronic Demyelinating Mouse Model. Article Snippet: Demyelinating diseases including multiple sclerosis (MS) are chronic inflammatory diseases of the central nervous system.. Indoleamine 2,3-dioxygenase 2 (Ido2) is a recently identified as catalytic enzyme involved in the rate-limiting step of the tryptophan-kynurenine pathway that influences susceptibility to inflammatory diseases.. However, the pathological role of Ido2 in demyelination remains unclear. Labeling:Article Title: CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction. Article Snippet: Although microglial activation is induced by an increase in chemokines, the role of mitophagy in this process remains unclear.. This study aimed to elucidate the role of microglial mitophagy in CKLF/CKLF1 (chemokine-like factor 1)-induced microglial activation and neuroinflammation, as well as the underlying molecular mechanisms following CKLF treatment.. This study determined that CKLF, an inducible chemokine in the brain, leads to an increase in mitophagy markers, such as DNM1L, PINK1 (PTEN induced putative kinase 1), PRKN, and OPTN, along with a simultaneous increase in autophagosome formation, as evidenced by elevated levels of BECN1 and MAP1LC3B (microtubule-associated protein 1 light chain 3 beta)-II. Staining:Article Title: Methodological framework for chromogenic mRNA detection using in situ hybridization chain reaction Article Snippet: .. The samples were washed with PBST three times for 5 min. For fluorescent staining, the amplification buffer was supplemented with Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions Article Snippet: For the quantification of Gr-1-positive cell areas in mouse lung tissue, lungs were fixed in 4% paraformaldehyde (PFA) and frozen embedded in Tissue-Tek OCT compound (Sakura Finetek Japan, Japan). .. Lung sections were reacted with anti-Gr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions. Article Snippet: .. Lung sections were reacted with antiGr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Amplification:Article Title: Methodological framework for chromogenic mRNA detection using in situ hybridization chain reaction Article Snippet: .. The samples were washed with PBST three times for 5 min. For fluorescent staining, the amplification buffer was supplemented with other:Article Title: Role of a novel endoplasmic reticulum-resident glycoprotein Mtc6/Ehg2 in high-pressure growth: stability of tryptophan permease Tat2 in Saccharomyces cerevisiae. Article Snippet: Deep-sea organisms are subjected to extreme conditions; therefore, understanding their adaptive strategies is crucial.. We utilize Saccharomyces cerevisiae as a model to investigate pressure-dependent protein regulation and piezo-adaptation.. Using yeast deletion library analysis, we identified 6 poorly characterized genes that are crucial for high-pressure growth, forming novel functional modules associated with cell growth. Article Title: Ladinin-1 in actin arcs of oral squamous cell carcinoma is involved in cell migration and epithelial phenotype Article Snippet: Immunofluorescent images using phalloidin (InvitrogenTM) for F-actin and Microscopy:Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions Article Snippet: For the quantification of Gr-1-positive cell areas in mouse lung tissue, lungs were fixed in 4% paraformaldehyde (PFA) and frozen embedded in Tissue-Tek OCT compound (Sakura Finetek Japan, Japan). .. Lung sections were reacted with anti-Gr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions. Article Snippet: .. Lung sections were reacted with antiGr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Laser-Scanning Microscopy:Article Title: TRIM47-CDO1 axis dictates hepatocellular carcinoma progression by modulating ferroptotic cell death through the ubiquitin‒proteasome system. Article Snippet: Hepatocellular carcinoma (HCC) is the predominant form of liver cancer, characterized by high morbidity and mortality rates, as well as unfavorable treatment outcomes.. Tripartite motif-containing protein 47 (TRIM47) has been implicated in various diseases including tumor progression with the activity of E3 ubiquitin ligase.. However, the precise regulatory mechanisms underlying the involvement of TRIM47 in HCC remain largely unexplored. |
