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hoechst 33 342  (Dojindo Labs)


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    Structured Review

    Dojindo Labs hoechst 33 342
    Hoechst 33 342, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoechst+33+342/Cellstain+Hoechst+33342+solution/pmc13149615-112-20-24
    Average 96 stars, based on 384 article reviews
    hoechst 33 342 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction.
    Article Snippet: Although microglial activation is induced by an increase in chemokines, the role of mitophagy in this process remains unclear.. This study aimed to elucidate the role of microglial mitophagy in CKLF/CKLF1 (chemokine-like factor 1)-induced microglial activation and neuroinflammation, as well as the underlying molecular mechanisms following CKLF treatment.. This study determined that CKLF, an inducible chemokine in the brain, leads to an increase in mitophagy markers, such as DNM1L, PINK1 (PTEN induced putative kinase 1), PRKN, and OPTN, along with a simultaneous increase in autophagosome formation, as evidenced by elevated levels of BECN1 and MAP1LC3B (microtubule-associated protein 1 light chain 3 beta)-II.

    Article Title: Ido2 Deficiency Exacerbates Motor Impairment and Reduces Aryl Hydrocarbon Receptor Activity through Decreased Kynurenine in a Chronic Demyelinating Mouse Model.
    Article Snippet: Demyelinating diseases including multiple sclerosis (MS) are chronic inflammatory diseases of the central nervous system.. Indoleamine 2,3-dioxygenase 2 (Ido2) is a recently identified as catalytic enzyme involved in the rate-limiting step of the tryptophan-kynurenine pathway that influences susceptibility to inflammatory diseases.. However, the pathological role of Ido2 in demyelination remains unclear.

    Labeling:

    Article Title: CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction.
    Article Snippet: Although microglial activation is induced by an increase in chemokines, the role of mitophagy in this process remains unclear.. This study aimed to elucidate the role of microglial mitophagy in CKLF/CKLF1 (chemokine-like factor 1)-induced microglial activation and neuroinflammation, as well as the underlying molecular mechanisms following CKLF treatment.. This study determined that CKLF, an inducible chemokine in the brain, leads to an increase in mitophagy markers, such as DNM1L, PINK1 (PTEN induced putative kinase 1), PRKN, and OPTN, along with a simultaneous increase in autophagosome formation, as evidenced by elevated levels of BECN1 and MAP1LC3B (microtubule-associated protein 1 light chain 3 beta)-II.

    Staining:

    Article Title: Methodological framework for chromogenic mRNA detection using in situ hybridization chain reaction
    Article Snippet: .. The samples were washed with PBST three times for 5 min. For fluorescent staining, the amplification buffer was supplemented with Hoechst 33,342 (1 μg/ml, Dojindo, Kumamoto, Japan; H342), and the slides were coverslipped with VECTASHIELD Vibrance (Vector Laboratory, Burlingame, CA, USA; H-1700). ..

    Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions
    Article Snippet: For the quantification of Gr-1-positive cell areas in mouse lung tissue, lungs were fixed in 4% paraformaldehyde (PFA) and frozen embedded in Tissue-Tek OCT compound (Sakura Finetek Japan, Japan). .. Lung sections were reacted with anti-Gr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Hoechst 33,342 (346–07951, Dojindo, Kumamoto, Japan), the slides were mounted and photographed using BZ-9000 microscope. ..

    Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions.
    Article Snippet: .. Lung sections were reacted with antiGr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Hoechst 33,342 (346–07951, Dojindo, Kumamoto, Japan), the slides were mounted and photographed using BZ-9000 microscope. ..

    Amplification:

    Article Title: Methodological framework for chromogenic mRNA detection using in situ hybridization chain reaction
    Article Snippet: .. The samples were washed with PBST three times for 5 min. For fluorescent staining, the amplification buffer was supplemented with Hoechst 33,342 (1 μg/ml, Dojindo, Kumamoto, Japan; H342), and the slides were coverslipped with VECTASHIELD Vibrance (Vector Laboratory, Burlingame, CA, USA; H-1700). ..

    other:

    Article Title: Role of a novel endoplasmic reticulum-resident glycoprotein Mtc6/Ehg2 in high-pressure growth: stability of tryptophan permease Tat2 in Saccharomyces cerevisiae.
    Article Snippet: Deep-sea organisms are subjected to extreme conditions; therefore, understanding their adaptive strategies is crucial.. We utilize Saccharomyces cerevisiae as a model to investigate pressure-dependent protein regulation and piezo-adaptation.. Using yeast deletion library analysis, we identified 6 poorly characterized genes that are crucial for high-pressure growth, forming novel functional modules associated with cell growth.

    Article Title: Ladinin-1 in actin arcs of oral squamous cell carcinoma is involved in cell migration and epithelial phenotype
    Article Snippet: Immunofluorescent images using phalloidin (InvitrogenTM) for F-actin and Hoechst 33,342 (1:200; Dojindo Laboratories Co., Ltd., Kumamoto, Japan) for nucleus on HSC-2, HSC-3, and HSC-4 were taken by BZ-9000 system (Keyence, Osaka, Japan).

    Microscopy:

    Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions
    Article Snippet: For the quantification of Gr-1-positive cell areas in mouse lung tissue, lungs were fixed in 4% paraformaldehyde (PFA) and frozen embedded in Tissue-Tek OCT compound (Sakura Finetek Japan, Japan). .. Lung sections were reacted with anti-Gr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Hoechst 33,342 (346–07951, Dojindo, Kumamoto, Japan), the slides were mounted and photographed using BZ-9000 microscope. ..

    Article Title: NOX2-Dependent Phagocyte Signaling Mediates Silica-Induced Murine Lung Injury via Macrophage-Neutrophil Interactions.
    Article Snippet: .. Lung sections were reacted with antiGr-1 antibody (ab25377, 1:200; Abcam, Cambridge, UK) overnight, and reacted with anti-rat biotin-conjugated IgG antibody (BA-9400; Vector Laboratories, Newark, CA, USA) at room temperature for 1 h. And then, the slides were washed with PBS, reacted with an Alexa Fluor 594-conjugated streptavidin antibody (S32356, 1:200; Invitrogen, Carlsbad, CA, USA) at room temperature for 1 h. After nuclear staining with Hoechst 33,342 (346–07951, Dojindo, Kumamoto, Japan), the slides were mounted and photographed using BZ-9000 microscope. ..

    Laser-Scanning Microscopy:

    Article Title: TRIM47-CDO1 axis dictates hepatocellular carcinoma progression by modulating ferroptotic cell death through the ubiquitin‒proteasome system.
    Article Snippet: Hepatocellular carcinoma (HCC) is the predominant form of liver cancer, characterized by high morbidity and mortality rates, as well as unfavorable treatment outcomes.. Tripartite motif-containing protein 47 (TRIM47) has been implicated in various diseases including tumor progression with the activity of E3 ubiquitin ligase.. However, the precise regulatory mechanisms underlying the involvement of TRIM47 in HCC remain largely unexplored.



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    Beyotime hoechst 33 342 staining solution
    FA suppresses OGD/R astrocyte pyroptosis and inflammation through hindering NLRP3 inflammasome activation. (A–B) <t>Hoechst</t> <t>33,342</t> and PI staining showed that 80 μM FA reduced pyroptosis in U251 cells after OGD/R, whereas NLRP3 agonist Nig resulted in increased pyroptosis (20×, 100 μm). (C) LDH assay showed that LDH release from U251 cells was increased following OGD/R FA inhibited LDH release, while Nig led to a rebound in LDH release. (D–E) Immunofluorescence co‐localization measured that OGD/R resulted in increased GSDMD‐N fluorescence intensity in U251 cells, FA treatment decreased GSDMD‐N level, and Nig attenuated the effect of FA (20×, 100 μm). (F–I) ELISA indicated that OGD/R resulted in increased IL‐1β, TNF‐α, IL‐18, and IL‐6 levels; FA treatment declined these inflammatory factors, but addition of Nig resulted in increased levels. (J–M) Western blot measured that OGD/R caused elevated iNOS, IL‐1β, and GSDMD‐N levels in U251 cells, and FA treatment decreased these protein levels, whereas the addition of Nig caused a rebound in these protein levels. (N) SEM to observe the morphological changes associated with pyroptosis in U251 cells. *** p < 0.001 vs. Control; ** p < 0.01 vs. OGD/R; && p < 0.01 vs. 80 μM FA.
    Hoechst 33 342 Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime hoechst 33 342 solution
    FA suppresses OGD/R astrocyte pyroptosis and inflammation through hindering NLRP3 inflammasome activation. (A–B) <t>Hoechst</t> <t>33,342</t> and PI staining showed that 80 μM FA reduced pyroptosis in U251 cells after OGD/R, whereas NLRP3 agonist Nig resulted in increased pyroptosis (20×, 100 μm). (C) LDH assay showed that LDH release from U251 cells was increased following OGD/R FA inhibited LDH release, while Nig led to a rebound in LDH release. (D–E) Immunofluorescence co‐localization measured that OGD/R resulted in increased GSDMD‐N fluorescence intensity in U251 cells, FA treatment decreased GSDMD‐N level, and Nig attenuated the effect of FA (20×, 100 μm). (F–I) ELISA indicated that OGD/R resulted in increased IL‐1β, TNF‐α, IL‐18, and IL‐6 levels; FA treatment declined these inflammatory factors, but addition of Nig resulted in increased levels. (J–M) Western blot measured that OGD/R caused elevated iNOS, IL‐1β, and GSDMD‐N levels in U251 cells, and FA treatment decreased these protein levels, whereas the addition of Nig caused a rebound in these protein levels. (N) SEM to observe the morphological changes associated with pyroptosis in U251 cells. *** p < 0.001 vs. Control; ** p < 0.01 vs. OGD/R; && p < 0.01 vs. 80 μM FA.
    Hoechst 33 342 Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoechst+33+342/Hoechst+33342+Staining+Solution/pmc13099128-79-5-8
    Average 99 stars, based on 1 article reviews
    hoechst 33 342 solution - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    FA suppresses OGD/R astrocyte pyroptosis and inflammation through hindering NLRP3 inflammasome activation. (A–B) Hoechst 33,342 and PI staining showed that 80 μM FA reduced pyroptosis in U251 cells after OGD/R, whereas NLRP3 agonist Nig resulted in increased pyroptosis (20×, 100 μm). (C) LDH assay showed that LDH release from U251 cells was increased following OGD/R FA inhibited LDH release, while Nig led to a rebound in LDH release. (D–E) Immunofluorescence co‐localization measured that OGD/R resulted in increased GSDMD‐N fluorescence intensity in U251 cells, FA treatment decreased GSDMD‐N level, and Nig attenuated the effect of FA (20×, 100 μm). (F–I) ELISA indicated that OGD/R resulted in increased IL‐1β, TNF‐α, IL‐18, and IL‐6 levels; FA treatment declined these inflammatory factors, but addition of Nig resulted in increased levels. (J–M) Western blot measured that OGD/R caused elevated iNOS, IL‐1β, and GSDMD‐N levels in U251 cells, and FA treatment decreased these protein levels, whereas the addition of Nig caused a rebound in these protein levels. (N) SEM to observe the morphological changes associated with pyroptosis in U251 cells. *** p < 0.001 vs. Control; ** p < 0.01 vs. OGD/R; && p < 0.01 vs. 80 μM FA.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Forsythoside A Alleviates Neuroinflammatory Damage via Inhibiting TLR4 / NF ‐ κB / NLRP3 Activation‐Induced Astrocyte Pyroptosis in Cerebral Ischemia–Reperfusion

    doi: 10.1002/cns.70867

    Figure Lengend Snippet: FA suppresses OGD/R astrocyte pyroptosis and inflammation through hindering NLRP3 inflammasome activation. (A–B) Hoechst 33,342 and PI staining showed that 80 μM FA reduced pyroptosis in U251 cells after OGD/R, whereas NLRP3 agonist Nig resulted in increased pyroptosis (20×, 100 μm). (C) LDH assay showed that LDH release from U251 cells was increased following OGD/R FA inhibited LDH release, while Nig led to a rebound in LDH release. (D–E) Immunofluorescence co‐localization measured that OGD/R resulted in increased GSDMD‐N fluorescence intensity in U251 cells, FA treatment decreased GSDMD‐N level, and Nig attenuated the effect of FA (20×, 100 μm). (F–I) ELISA indicated that OGD/R resulted in increased IL‐1β, TNF‐α, IL‐18, and IL‐6 levels; FA treatment declined these inflammatory factors, but addition of Nig resulted in increased levels. (J–M) Western blot measured that OGD/R caused elevated iNOS, IL‐1β, and GSDMD‐N levels in U251 cells, and FA treatment decreased these protein levels, whereas the addition of Nig caused a rebound in these protein levels. (N) SEM to observe the morphological changes associated with pyroptosis in U251 cells. *** p < 0.001 vs. Control; ** p < 0.01 vs. OGD/R; && p < 0.01 vs. 80 μM FA.

    Article Snippet: Hoechst 33,342 staining solution (C1025, Beyotime) was introduced and cultured for half an hour at 4°C.

    Techniques: Activation Assay, Staining, Lactate Dehydrogenase Assay, Immunofluorescence, Fluorescence, Enzyme-linked Immunosorbent Assay, Western Blot, Control